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KMID : 1025520040460030301
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Journal of Animal Science and Technology
2004 Volume.46 No. 3 p.301 ~ p.306
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Development of Microsatellite Markers using BAC clone Sequencing on Porcine Chromosome 6q28-6q32
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Jang Gul-Won
Kim Tae-Hun
Oh S.-J.
Cheong I. C.
Park Eung-Woo
Choi Bong-Hwan
Lee K. T.
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Abstract
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This study was conducted to develop new markers at the region that was related to QTL affecting intramuscular fat and backfat thickness on chromosome 6q28-6q32 in pigs. Dozens of repeated sequences were founded using shotgun sequencing of several BAC clones corresponding to that region, of which five new microstellite markers that identified polymorphism were discovered. The mean number of alleles at each locus observed 2.13(KP0290F2), 4.63(KP0248C11), 7.38(KP1231C91), 2.75(KP1231C92) and 6.25(KP1231C93) in 8 breeds(Landrace, Korean native pig, Duroc, Yorkshire, Berkshire, Wuzhishan pig, Xiang pig, Min pig). The average estimated heterozygosity values at each locus varied from 0.2100(KP0290F2) to 0.8304(KP1231C91) in all populations. In other hand, the average allele of all loci was within range of 0.4517(Berkshire) and 0.6957 (Yorkshire). Of these markers, KP0248C11, KP1231C91 and KP1231C93 were identified to have optimal number of alleles, high heterozygosity values and low standard deviation values. Especially, KP1231C91 and KP1231C93 might be considered as a useful marker for genetic mapping and diversity study.
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KEYWORD
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Microsatellite marker, Shotgun sequencing, Polymorphism, Heterozygosity, BAC, pig
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